Induction of a mutant phenotype in human repair proficient cells after overexpression of a mutated human DNA repair gene.

doi:10.1093/nar/19.20.5633

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Article

Induction of a mutant phenotype in human repair proficient cells after overexpression of a mutated human DNA repair gene.

Nucleic acids research - 25 Oct 1991

Belt P B, van Oosterwijk M F, Odijk H, Hoeijmakers J H, Backendorf C

Abstract excerpt

Antisense and mutated cDNA of the human excision repair gene ERCC-1 were overexpressed in repair proficient HeLa cells by means of an Epstein-Barr-virus derived cDNA expression vector. Whereas antisense RNA did not influence the survival of the transfected cells, a mutated cDNA generating an ERCC-1 protein with two extra amino acids in a conserved region of its C-terminal part resulted in a significant...

Topics

Amino Acid Sequence
Animals
Cloning, Molecular
DNA Repair
DNA-Binding Proteins
Endonucleases
Gene Expression Regulation
HeLa Cells
Humans
Mice
Mitomycin

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Mutation

Phenotype

Proteins

RNA, Antisense

Sequence Alignment

Transfection

Source

Open in PubMed Open full text

Identifiers

PMID: 1945841
DOI: 10.1093/nar/19.20.5633
PMCID: PMC328968

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1945841-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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