Article
Mutagenic DNA repair in Escherichia coli, XX. Overproduction of UmuD' protein results in suppression of the umuC36 mutation in excision defective bacteria.
Mutation research - 1 Jan 2000
Bates H, Bridges B A, Woodgate R
Abstract excerpt
Overproduction of Umu+ or UmuD' protein by means of a gene carried on a multicopy plasmid suppressed the umuC36 phenotype and permitted induction of mutations by ultraviolet light. The umuC122::Tn5 phenotype was not suppressed. Suppression of the umuC36 phenotype was only seen when excision repair was blocked by acriflavine or by an uvrA or uvrB mutation. Cleavage of UmuD to UmuD' in SOS-induced cells was not...
Topics
- Bacterial Proteins
- Blotting, Western
- DNA Repair
- DNA-Directed DNA Polymerase
- Escherichia coli
- Escherichia coli Proteins
- Histidine
- Mutation
- Plasmids
- Suppression, Genetic
- Ultraviolet Rays