Article
New mutations in cloned Escherichia coli umuDC genes: novel phenotypes of strains carrying a umuC125 plasmid.
Mutation research - 1 Jan 2000
Marsh L, Nohmi T, Hinton S, Walker G C
Abstract excerpt
The umuDC locus of Escherichia coli is required for most mutagenesis by UV and many chemicals. Mutations in E. coli umuDC genes cloned on pBR322-derived plasmids were isolated by two methods. First, spontaneously-arising mutant umuDC plasmids that failed to confer cold-sensitive growth on a lexA51(Def) strain were isolated by selection. Second, mutant umuDC plasmids that affected apparent mutant yield after...
Topics
- Alleles
- Cloning, Molecular
- Escherichia coli
- Genes, Bacterial
- Kinetics
- Mitomycin
- Mutagenesis
- Mutation
- Phenotype
- Plasmids
- SOS Response, Genetics
- Temperature