Article
Application of COLD-PCR for improved detection of KRAS mutations in clinical samples.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc - 1 Aug 2009
Zuo Zhuang, Chen Su S, Chandra Pranil K, Galbincea John M, Soape Matthew, Doan Steven, Barkoh Bedia A, Koeppen Hartmut, Medeiros L Jeffrey, Luthra Rajyalakshmi
Abstract excerpt
KRAS mutations have been detected in approximately 30% of all human tumors, and have been shown to predict response to some targeted therapies. The most common KRAS mutation-detection strategy consists of conventional PCR and direct sequencing. This approach has a 10-20% detection sensitivity depending on whether pyrosequencing or Sanger sequencing is used. To improve detection sensitivity, we compared our...
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