Article
Increased sensitivity of KRAS mutation detection by high-resolution melting analysis of COLD-PCR products.
Human mutation - 1 Dec 2010
Kristensen Lasse S, Daugaard Iben L, Christensen Mariann, Hamilton-Dutoit Stephen, Hager Henrik, Hansen Lise Lotte
Abstract excerpt
Considerable effort has been invested in the development of sophisticated technologies enabling detection of clinically significant low-level tumor specific KRAS mutations. Coamplification at lower denaturation temperature-PCR (COLD-PCR) is a new form of PCR that selectively amplifies mutation-containing templates based on the lower melting temperature of mutant homoduplexes versus wild-type homoduplexes. We have...
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