Article
An optimized method for the induction and purification of mouse bone marrow dendritic cells.
Journal of immunological methods - 1 Aug 2021
Liu Ling, Fan Shanwen, Lu Zhonghua, Chen Zhenxing, Chu Cuilin, Liu Airan, Xia Feiping, Meng Shanshan, Guo Fengmei, Qiu Haibo, Yang Yi
Abstract excerpt
Dendritic cells (DCs) play an essential role in the initiation of adaptive immune responses, but they are rare in all organs. The traditional methods used to increase the yield and purity of DCs are the early removal of granulocyte culture medium and the isolation of high-purity DCs by magnetic-activated cell sorting (MACS). This study provides a more rapid and economical optimization method to obtain more...
Topics
- Adaptive Immunity
- Animals
- Biomarkers
- Bone Marrow Cells
- Cell Proliferation
- Cell Separation
- Cells, Cultured
- Coculture Techniques
- Cost Savings
- Cost-Benefit Analysis
- Dendritic Cells
- Lymphocyte Activation
- Male