Elucidating and minimizing the loss by recombinant vaccinia virus of human immunodeficiency virus gene expression resulting from spontaneous mutations and positive selection.

Abstract excerpt

While characterizing modified vaccinia virus recombinants (rMVAs) containing human immunodeficiency virus env and gag-pol genes, we detected nonexpressing mutants by immunostaining individual plaques. In many cases, the numbers of mutants increased during successive passages, indicating strong selection pressure. This phenomenon provided an opportunity to investigate the formation of spontaneous mutations in...

Topics

• Base Sequence
• Cells, Cultured
• Fusion Proteins, gag-pol
• Gene Expression
• Genetic Vectors
• HIV
• HIV Infections
• Humans
• Molecular Sequence Data
• Mutation