Article
Enhanced cell surface expression, immunogenicity and genetic stability resulting from a spontaneous truncation of HIV Env expressed by a recombinant MVA.
Virology - 15 Mar 2008
Wyatt Linda S, Belyakov Igor M, Earl Patricia L, Berzofsky Jay A, Moss Bernard
Abstract excerpt
During propagation of modified vaccinia virus Ankara (MVA) encoding HIV 89.6 Env, a few viral foci stained very prominently. Virus cloned from such foci replicated to higher titers than the parent and displayed enhanced genetic stability on passage. Sequence analysis showed a single nucleotide deletion in the 89.6 env gene of the mutant that caused a frame shift and truncation of 115 amino acids from the...
Topics
- AIDS Vaccines
- Animals
- Antibodies, Viral
- Cell Line
- Cell Membrane
- Chick Embryo
- Female
- Fibroblasts
- Gene Expression Regulation, Viral
- HIV
- Immunoglobulin A
- Immunoglobulin G
- Mice
- Mice, Inbred BALB C