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Article

Isolation of variants of Chinese hamster ovary cells with abnormally low levels of GSH: decreased ability to cleave endocytosed disulfide bonds.

Journal of cellular physiology - 1 Oct 1991

Mandel R, Ryser H J, Niaki B, Ghani F, Shen W C

Abstract excerpt

Mutants of Chinese hamster ovary cells were selected for resistance to a 3 hour exposure to 4 microgram/ml N-methyl-N'-nitro-N-nitrosoguanidine and tested for glutathione (GSH) levels. Six of eight clones that survived the initial treatment had reduced GSH levels ranging from 26 to 61% of wild-type values. These eight cell lines were tested for their susceptibility to a drug conjugate in which methotrexate (MTX)...

Topics

Animals
CHO Cells
Cell Division
Cricetinae
Diphtheria Toxin
Disulfides
Drug Resistance
Endocytosis
Genetic Variation
Glutathione
Melphalan

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Methotrexate

Methylnitronitrosoguanidine

Mutation

Polylysine

Ricin

Source

Identifiers

PMID: 1939347
DOI: 10.1002/jcp.1041490109

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1939347-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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