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Article

Directed evolution of an extremely stable fluorescent protein.

Protein engineering, design & selection : PEDS - 1 May 2009

Kiss Csaba, Temirov Jamshid, Chasteen Leslie, Waldo Geoffrey S, Bradbury Andrew R M

Abstract excerpt

In this paper we describe the evolution of eCGP123, an extremely stable green fluorescent protein based on a previously described fluorescent protein created by consensus engineering (CGP: consensus green protein). eCGP123 could not be denatured by a standard thermal melt, preserved almost full fluorescence after overnight incubation at 80 degrees C and possessed a free energy of denaturation of 12.4 kcal/mol. It...

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Identifiers

PMID: 19364809
DOI: 10.1093/protein/gzp006

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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