Article
Experimental identification and theoretical analysis of a thermally stabilized green fluorescent protein variant.
Biochemistry - 9 Oct 2012
Akiyama Saori, Suenaga Atsushi, Kobayashi Takayuki, Kamioka Tetsuya, Taiji Makoto, Kuroda Yutaka
Abstract excerpt
In this study, we aim to relate experimentally measured macroscopic properties to dynamic and structural changes as calculated by molecular dynamics (MD) simulations. We performed the analysis on four GFP (green fluorescent protein) variants, which have amino acid replacements or insertion in a flexible region on the protein surface and which resulted from a previous protein splicing reaction optimization...
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