Article
New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria.
Applied and environmental microbiology - 1 Jun 1998
Andersen J B, Sternberg C, Poulsen L K, Bjorn S P, Givskov M, Molin S
Abstract excerpt
Use of the green fluorescent protein (Gfp) from the jellyfish Aequorea victoria is a powerful method for nondestructive in situ monitoring, since expression of green fluorescence does not require any substrate addition. To expand the use of Gfp as a reporter protein, new variants have been constr...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- DNA Primers
- Escherichia coli
- Gene Expression
- Genes, Reporter
- Genetic Variation
- Green Fluorescent Proteins
- Half-Life
- Luminescent Proteins
- Molecular Sequence Data
- Pseudomonas putida
- Recombinant Proteins
- Scyphozoa