Article
New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria.
Applied and environmental microbiology - 1 Jun 1998
Andersen J B, Sternberg C, Poulsen L K, Bjorn S P, Givskov M, Molin S
Abstract excerpt
Use of the green fluorescent protein (Gfp) from the jellyfish Aequorea victoria is a powerful method for nondestructive in situ monitoring, since expression of green fluorescence does not require any substrate addition. To expand the use of Gfp as a reporter protein, new variants have been constructed by the addition of short peptide sequences to the C-terminal end of intact Gfp. This rendered the Gfp susceptible...
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