Article
Sequences necessary for trans-splicing in transiently transfected Brugia malayi.
Molecular and biochemical parasitology - 1 Nov 2007
Liu Canhui, de Oliveira Ana, Higazi Tarig B, Ghedin Elodie, DePasse Jay, Unnasch Thomas R
Abstract excerpt
Many genes in parasitic nematodes are both cis- and trans-spliced. Previous studies have demonstrated that a 7nt element encoded in the first intron of the Brugia malayi 70kDa heat shock protein (BmHSP70) gene was necessary to permit trans-splicing of transgenic mRNAs in embryos transfected with constructs encoding portions of the BmHSP70 gene. Here we demonstrate that this element (the B. malayi HSP70...
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