[The colocalized relationship between p53 mutants 249M, 273H and hsp70 in HepG2 cells].

Abstract excerpt

AIM: To synthesize p53 mutants 249M, 273H with site-directed mutagenesis and construct the relative GFP expression vectors, transfect HepG2 cells and then detect the co-localization of p53 and hsp70. METHODS: Two sets of primers were designed according to the gene sequence of p53, and mismatches were introduced into primers. Mutagenesis was performed in a two-step PCR. The amplified fragments containing the...

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