Article
[The expression and localization of wild-type p53-GFP fused gene on human high-metastasis hepatocellular carcinoma cell line].
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology - 1 Apr 2000
He P, Tang Z, Ye S
Abstract excerpt
OBJECTIVE: To study the normal expression and location of wt-p53 protein on human high-metastasis hepatocellular carcinoma (HCC) cell line. METHODS: According to the sequence of human wild-type p53, the mp53 cDNA in which stopped codon TGA was mutated to TGG, was amplified by PCR, ligated to EGFP gene, of pEGFP-N1. The recombinant plasmid was transfected into MHCC97, in which p53 gene was mutated. The expression...
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