Article
Structural stability of green fluorescent proteins entrapped in polyelectrolyte nanocapsules.
Journal of biophotonics - 1 Sept 2008
Krol Silke, Cannone Fabio, Caccia Michele, Sironi Laura, Bianchini Paolo, Campanini Barbara, Collini Maddalena, Chirico Giuseppe, Diaspro Alberto
Abstract excerpt
Molecules of a green fluorescent protein mutant, GFPmut2, have been immobilized in nanocapsules, assemblies of charged polyelectrolyte multilayers, with the aim to study the effect of protein-polyelectrolyte interactions on the protein stability against chemical denaturation. GFPmut2 proteins turn out to be stabilized and protected against the denaturating action of small chemical compounds. The nanocapsule...
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