Article
Voltage regulation of single green fluorescent protein mutants.
Biophysical chemistry - 1 Feb 2007
Cannone Fabio, Milani Roberto, Chirico Giuseppe, Diaspro Alberto, Krol Silke, Campanini Barbara
Abstract excerpt
We report the analysis of the fluorescence intensity fluctuations of single proteins of a GFP mutant, GFPmut2, embedded in a polyelectrolyte nanocapsule adsorbed on thin conductive layers. Our results, based on single molecule fluorescence spectroscopy, indicate that the fluorescence blinking dynamics of GFP is strongly dependent on the bulk conductivity of the metal layer substrate, on the distance from the...
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