Article
High-throughput screening of catalytically inactive mutants of protein tyrosine phosphatases (PTPs) in a phosphopeptide microarray.
Chemical communications (Cambridge, England) - 14 Feb 2009
Sun Hongyan, Tan Lay Pheng, Gao Liqian, Yao Shao Q
Abstract excerpt
We report herein a novel phosphopeptide microarray capable of noncovalently "trapping" catalytically inactive mutants of protein tyrosine phosphatases (PTPs), and its application in high-throughput determination of PTP substrate specificity.
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