Article

High-throughput biochemical phenotyping of SHP2 variants reveals the molecular basis of diseases and allosteric drug inhibition

2026-04-01

Abstract excerpt

Interpreting clinical and functional consequences of genetic variants remains challenging due to limited quantitative biochemical data at scale. We applied high-throughput microfluidic enzyme kinetics to profile 190 clinical variants of SHP2, a protein tyrosine phosphatase linked to developmental disorders and cancers. Through >300,000 reaction progress curves, we derived kinetic and thermodynamic parameters quant...

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Literature Corpus work: 23e91a52-d916-5f62-b7d7-77621f616f4f
DOI: 10.64898/2026.03.30.715055

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