Article
Stability and sequence-specific DNA binding of activation-labile mutants of the human glucocorticoid receptor.
Biochemistry - 19 Nov 1991
Elsasser M S, Eisen L P, Riegel A T, Harmon J M
Abstract excerpt
The stability and DNA-binding properties of activation-labile (act1) human glucocorticoid receptors (hGRs) from the glucocorticoid-resistant mutant 3R7.6TG.4 were investigated. These receptors are able to bind reversibly associating ligands with normal affinity and specificity, but become unstable during attempted activation to the DNA binding form [Harmon et al. (1984) J. Steroid Biochem. 21, 227-236]. Affinity...
Topics
- Base Sequence
- Cell Line
- DNA
- DNA-Binding Proteins
- Humans
- Molecular Sequence Data
- Mutation
- Receptors, Glucocorticoid
- Transcriptional Activation