Article
Active, interactive, and inactive steroid receptor mutants.
Steroids - 1 Feb 1994
Lanz R B, Hug M, Gola M, Tallone T, Wieland S, Rusconi S
Abstract excerpt
In transient co-transfection assays, there is extensive cross-interaction between glucocorticoid receptor (GR) domains. For example, mutation of the conserved Ile residue at position 484 (rat GR map) to cysteine allows a net separation of transactivation and DNA binding. We also observed that the ligand binding domain plays a key role in cooperative transactivation. Furthermore, some carboxy-located mutations...
Topics
- Amino Acid Sequence
- Base Sequence
- Binding Sites
- DNA
- Enhancer Elements, Genetic
- Molecular Sequence Data
- Mutation
- Promoter Regions, Genetic
- Receptors, Glucocorticoid
- Repetitive Sequences, Nucleic Acid
- Transcriptional Activation