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Article

Preparation of heteroduplex enhanced green fluorescent protein plasmid for in vivo mismatch repair activity assay.

Analytical biochemistry - 1 May 2009

Zhou Bisheng, Huang Changjiang, Yang Junhua, Lu Jianxin, Dong Qiaoxiang, Sun Lu-Zhe

Abstract excerpt

Preparation of heteroduplexes in large quantities with high purity is essential for the measurement of DNA mismatch repair (MMR) activity. Here we report a rapid, less labor-intensive method for the preparation of a heteroduplex plasmid that expresses the enhanced green fluorescent protein (EGFP) if the mismatch is repaired correctly. The method involves the use of a wild-type and a mutated EGFP expression...

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Identifiers

PMID: 19248754
DOI: 10.1016/j.ab.2009.02.020
PMCID: PMC2670966

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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