Detection of T:T mismatch base pair by fluorescence spectral change upon addition of mercury (II) cation: toward the efficient analyses of single nucleotide polymorphism.

Abstract excerpt

We have already found that mercury (II) cation specifically binds to T:T mismatch base pair in heteroduplex, which increases the melting temperature of heteroduplex involving T:T mismatch base pair by about 4 degrees C. This result shows that addition of the metal cation is a promising strategy for the mismatch base pair detection in heteroduplex, but UV melting to determine the melting temperature is...

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