Flow cytometric detection of tandem repeat mutations induced by various chemical classes.

doi:10.1016/j.mrfmmm.2006.01.012

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Article

Flow cytometric detection of tandem repeat mutations induced by various chemical classes.

Mutation research - 25 Jun 2006

Healy Caroline, Wade Mike, McMahon Avril, Williams Andrew, Johnson Douglas A, Parfett Craig

Abstract excerpt

To facilitate detection of genotoxicity from environmental mutagen exposure, we generated an in vitro enhanced green fluorescence protein (EGFP) reactivation assay that quickly and effectively detects frameshift mutations in tandem repeat sequences (TRS). Two murine cell lines, C3H10T1/2 and mism...

Topics

Animals
Base Sequence
Blotting, Northern
Cells, Cultured
DNA Primers
Flow Cytometry
Genes, Reporter
Green Fluorescent Proteins
Mice
Mice, Inbred C3H
Mutagens
Mutation
RNA
Tandem Repeat Sequences

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