Article
Isolation and characterization of PEP3, a gene required for vacuolar biogenesis in Saccharomyces cerevisiae.
Molecular and cellular biology - 1 Dec 1991
Preston R A, Manolson M F, Becherer K, Weidenhammer E, Kirkpatrick D, Wright R, Jones E W
Abstract excerpt
The Saccharomyces cerevisiae PEP3 gene was cloned from a wild-type genomic library by complementation of the carboxypeptidase Y deficiency in a pep3-12 strain. Subclone complementation results localized the PEP3 gene to a 3.8-kb DNA fragment. The DNA sequence of the fragment was determined; a 2,7...
Topics
- Adaptor Proteins, Vesicular Transport
- Alleles
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- DNA, Fungal
- Fungal Proteins
- Genes, Fungal
- Homozygote
- Hot Temperature
- Hydrolases
- Immunoblotting
- Membrane Proteins
- Molecular Sequence Data