Article
Direct localization of a beta-subunit domain on the three-dimensional structure of Escherichia coli RNA polymerase.
Proceedings of the National Academy of Sciences of the United States of America - 18 Jan 2000
Opalka N, Mooney R A, Richter C, Severinov K, Landick R, Darst S A
Abstract excerpt
To identify the location of a domain of the beta-subunit of Escherichia coli RNA polymerase (RNAP) on the three-dimensional structure, we developed a method to tag a nonessential surface of the multisubunit enzyme with a protein density easily detectable by electron microscopy and image processing. Four repeats of the IgG-binding domain of Staphylococcus aureus protein A were inserted at position 998 of the E....
Topics
- Crystallization
- Crystallography, X-Ray
- DNA-Directed RNA Polymerases
- Escherichia
- Microscopy, Electron
- Mutation
- Protein Conformation
- Protein Structure, Tertiary