Article
Visualization and functional analysis of RNA-dependent RNA polymerase lattices.
Science (New York, N.Y.) - 21 Jun 2002
Lyle John M, Bullitt Esther, Bienz Kurt, Kirkegaard Karla
Abstract excerpt
Positive-strand RNA viruses such as poliovirus replicate their genomes on intracellular membranes of their eukaryotic hosts. Electron microscopy has revealed that purified poliovirus RNA-dependent RNA polymerase forms planar and tubular oligomeric arrays. The structural integrity of these arrays correlates with cooperative RNA binding and RNA elongation and is sensitive to mutations that disrupt intermolecular...
Topics
- Base Sequence
- Binding Sites
- Catalysis
- Crystallography, X-Ray
- HeLa Cells
- Humans
- Hydrogen-Ion Concentration
- Inclusion Bodies, Viral
- Microscopy, Electron
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Poliovirus