Article
Identification of a residue critical for the excision of 3'-blocking ends in apurinic/apyrimidinic endonucleases of the Xth family.
Nucleic acids research - 1 Apr 2009
Castillo-Acosta Víctor M, Ruiz-Pérez Luis M, Yang Wei, González-Pacanowska Dolores, Vidal Antonio E
Abstract excerpt
DNA single-strand breaks containing 3'-blocking groups are generated from attack of the sugar backbone by reactive oxygen species or after base excision by DNA glycosylase/apurinic/apyrimidinic (AP) lyases. In human cells, APE1 excises sugar fragments that block the 3'-ends thus facilitating DNA repair synthesis. In Leishmania major, the causal agent of leishmaniasis, the APE1 homolog is the class II AP...
Topics
- Amino Acid Sequence
- Amino Acid Substitution
- Animals
- DNA-(Apurinic or Apyrimidinic Site) Lyase
- Escherichia coli
- Exonucleases
- Hydrogen Peroxide
- Leishmania major
- Magnesium
- Molecular Sequence Data