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Article

Noncatalytic aspartate at the exonuclease domain of proofreading DNA polymerases regulates both degradative and synthetic activities.

Proceedings of the National Academy of Sciences of the United States of America - 27 Mar 2018

Del Prado Alicia, Franco-Echevarría Elsa, González Beatriz, Blanco Luis, Salas Margarita, de Vega Miguel

Abstract excerpt

Most replicative DNA polymerases (DNAPs) are endowed with a 3'-5' exonuclease activity to proofread the polymerization errors, governed by four universally conserved aspartate residues belonging to the Exo I, Exo II, and Exo III motifs. These residues coordinate the two metal ions responsible for the hydrolysis of the last phosphodiester bond of the primer strand. Structural alignment of the conserved exonuclease...

Topics

Amino Acid Sequence
Aspartic Acid
Bacillus Phages
DNA Repair
DNA Replication
DNA-Directed DNA Polymerase
Exodeoxyribonucleases
Mutagenesis, Site-Directed
Mutation
Sequence Homology

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Identifiers

PMID: 29531047
DOI: 10.1073/pnas.1718787115
PMCID: PMC5879684

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