Article
Transcription from a bacteriophage T4 middle promoter using T4 motA protein and phage-modified RNA polymerase.
The Journal of biological chemistry - 25 Sept 1991
Hinton D M
Abstract excerpt
The bacteriophage T4 motA protein is required for transcription from T4 middle promoters. These promoters, which contain the Escherichia coli promoter consensus sequence at the -10 region (TATAAT) but a unique sequence centered at -30 ((a/t)(a/t)TGCTT(t/c)A) (Guild, N., Gayle, M., Sweeney, R., Hollingsworth, T., Modeer, T., and Gold, L. (1988) J. Mol. Biol. 199, 241-258), become active about 2 min after...
Topics
- Base Sequence
- Cell Line
- DNA, Viral
- DNA-Binding Proteins
- DNA-Directed RNA Polymerases
- Electrophoresis, Polyacrylamide Gel
- Genes, Viral
- Molecular Sequence Data
- Mutation
- Plasmids
- Promoter Regions, Genetic
