Article
Simple and rapid genotyping of D-amino acid oxidase gene recognizing a crucial variant in the ddY strain using microchip electrophoresis.
Journal of separation science - 1 Feb 2009
Tojo Yosuke, Hamase Kenji, Konno Ryuichi, Koyanagi Satoru, Ohdo Shigehiro, Zaitsu Kiyoshi
Abstract excerpt
A rapid genotyping method of D-amino acid oxidase (DAO), an enzyme that catalyzes the oxidative degradation of most of the D-amino acids in mammals, has been established. This method employs a one-step PCR, restriction enzyme digestion and rapid microchip electrophoresis (MCE), and the DAO genotype of the living individual mice was definitely determined within a day by clearly separating the 95 and 107 bp Hpa II...
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