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Article

Genetic transformation of auxotrophic mutants of the filamentous yeast Trichosporon cutaneum using homologous and heterologous marker genes.

Yeast (Chichester, England) - 1 Jul 1991

Ochsner U A, Glumoff V, Kälin M, Fiechter A, Reiser J

Abstract excerpt

A transformation system for the filamentous yeast Trichosporon cutaneum based on auxotrophic markers is presented and techniques for the induction, isolation and characterization of mutants are described. A number of auxotrophic mutants were isolated and characterized by using biosynthetic precursors and/or inhibitors. A mutant unable to grow in the presence of ornithine could be complemented successfully by...

Topics

Arginine
Blotting, Southern
Cloning, Molecular
Genes, Fungal
Genetic Complementation Test
Genetic Markers
Mutation
Ornithine Carbamoyltransferase
Transformation, Genetic
Trichosporon

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 1897316
DOI: 10.1002/yea.320070511

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1897316-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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