Identification of the orotidine-5'-phosphate decarboxylase gene and development of a transformation system in the yeast Saccharomyces exiguus Yp74L-3.

doi:10.1271/bbb.62.2280

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Article

Identification of the orotidine-5'-phosphate decarboxylase gene and development of a transformation system in the yeast Saccharomyces exiguus Yp74L-3.

Bioscience, biotechnology, and biochemistry - 1 Nov 1998

Hisatomi T, Kuroyanagi S, Tsuboi M

Abstract excerpt

To investigate the uracil biosynthetic pathway of the yeast Saccharomyces exiguus Yp74L-3, uracil auxotrophic mutants were isolated. Using conventional genetic techniques, four mutant genes concerned in uracil biosynthesis were identified and denoted as ura1, ura2, ura3, and ura4. Mutations in th...

Topics

Genes, Fungal
Genetic Vectors
Mutation
Orotic Acid
Orotidine-5'-Phosphate Decarboxylase
Plasmids
Saccharomyces
Transformation, Genetic
Uracil

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