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Article

A counter-selectable marker for genetic transformation of the yeast Schwanniomyces alluvius.

Applied microbiology and biotechnology - 1 Aug 1997

Dave M N, Chattoo B B

Abstract excerpt

We report here a counter-selectable marker system for genetic transformation of the yeast Schwanniomyces alluvius, based on the complementation of uracil auxotrophs defective in either orotidine-5'-phosphate decarboxylase (URA3) or orotidine-5'-pyrophosphate (URA5). Uracil auxotrophs of S. alluvius were obtained by ethyl methanesulphonate mutagenesis and complemented using the ura3 gene from S. cerevisiae. A...

Topics

Genetic Markers
Mitosis
Mutation
Saccharomycetales
Transformation, Genetic

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 9299778
DOI: 10.1007/s002530051039

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