Article
A counter-selectable marker for genetic transformation of the yeast Schwanniomyces alluvius.
Applied microbiology and biotechnology - 1 Aug 1997
Dave M N, Chattoo B B
Abstract excerpt
We report here a counter-selectable marker system for genetic transformation of the yeast Schwanniomyces alluvius, based on the complementation of uracil auxotrophs defective in either orotidine-5'-phosphate decarboxylase (URA3) or orotidine-5'-pyrophosphate (URA5). Uracil auxotrophs of S. alluvius were obtained by ethyl methanesulphonate mutagenesis and complemented using the ura3 gene from S. cerevisiae. A...
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