Article
Positive modulation of RNA polymerase III transcription by ribosomal proteins.
Biochemical and biophysical research communications - 6 Feb 2009
Dieci Giorgio, Ruotolo Roberta, Braglia Priscilla, Carles Christophe, Carpentieri Andrea, Amoresano Angela, Ottonello Simone
Abstract excerpt
A yeast nuclear fraction of unknown composition, named TFIIIE, was reported previously to enhance transcription of tRNA and 5S rRNA genes in vitro. We show that TFIIIE activity co-purifies with a specific subset of ribosomal proteins (RPs) which, as revealed by chromatin immunoprecipitation analysis, generally interact with tRNA and 5S rRNA genes, but not with a Pol II-specific promoter. Only Rpl6Ap and Rpl6Bp,...
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