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Article

Ligase detection reaction for the analysis of point mutations using free-solution conjugate electrophoresis in a polymer microfluidic device.

Electrophoresis - 1 Dec 2008

Sinville Rondedrick, Coyne Jennifer, Meagher Robert J, Cheng Yu-Wei, Barany Francis, Barron Annelise, Soper Steven A

Abstract excerpt

We have developed a new method for the analysis of low abundant point mutations in genomic DNA using a combination of an allele-specific ligase detection reaction (LDR) with free-solution conjugate electrophoresis (FSCE) to generate and analyze the genetic products. FSCE eliminates the need for a polymer sieving matrix by conjugating chemically synthesized polyamide "drag-tags" onto the LDR primers. The...

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Identifiers

PMID: 19053073
DOI: 10.1002/elps.200800197
PMCID: PMC3010182

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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