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Acoustic detection of a mutation-specific Ligase Chain Reaction based on liposome amplification.

The Analyst - 24 Jun 2024

Naoumi Nikoletta, Araya-Farias Monica, Megariti Maria, Alexandre Lucile, Papadakis George, Descroix Stephanie, Gizeli Electra

Abstract excerpt

Single nucleotide variants (SNVs) play a crucial role in understanding genetic diseases, cancer development, and personalized medicine. However, existing ligase-based amplification and detection techniques, such as Rolling Circle Amplification and Ligase Detection Reaction, suffer from low efficiency and difficulties in product detection. To address these limitations, we propose a novel approach that combines...

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PMID: 38758167
DOI: 10.1039/d3an02142d

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