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Article

Use of the native flp gene to generate in-frame unmarked mutations in Streptomyces spp.

Gene - 15 Aug 2009

Zelyas Nathan, Tahlan Kapil, Jensen Susan E

Abstract excerpt

The generation of unmarked deletion mutants free from polar effects on downstream genes is typically a lengthy and arduous process in Streptomyces spp. The use of FLP recombinase can greatly facilitate this process when combined with established polymerase chain reaction (PCR)-targeting techniques. In vivo production of FLP within Streptomyces cells would streamline the process further, but expression of flp in...

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PMID: 19446014
DOI: 10.1016/j.gene.2009.03.022

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