Article
Reverse transcription PCR-based sequence analysis of hepatitis C virus replicon RNA.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2009
Tellinghuisen Timothy L, Lindenbach Brett D
Abstract excerpt
Since the advent of efficient cell-culture methods for HCV replication and, more recently, infection, there has been a need to efficiently sequence the viral RNA in these systems. This need is especially urgent in light of the error-prone nature of HCV RNA replication, which leads to a variety of interesting mutations. The adaptation of hepatitis C replicons to cell culture, which greatly increased their...
Topics
- Cell Culture Techniques
- Cloning, Molecular
- DNA, Complementary
- Genome, Viral
- Genotype
- Hepacivirus
- Indicators and Reagents
- RNA
- RNA, Viral
- Replicon
- Reverse Transcriptase Polymerase Chain Reaction
- Reverse Transcription
- Sequence Analysis, DNA
- Species Specificity
- Virus Replication