Article
Mutant profiles of selectable genetic elements.
Proceedings of the National Academy of Sciences of the United States of America - 15 Nov 1991
Wurst H, Pohl F M
Abstract excerpt
A method is presented that allows simultaneous analysis of the effects of all possible point mutations within a specific mutation window of at least 50 base pairs on a DNA fragment that codes for a selectable function. It relies on the detection of mismatched base pairs with hydroxylamine and osm...
Topics
- Amino Acid Sequence
- Base Composition
- Base Sequence
- DNA, Bacterial
- Escherichia coli
- Gene Library
- Genetic Complementation Test
- Hydroxylamine
- Hydroxylamines
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Nucleic Acid Heteroduplexes
- Oligodeoxyribonucleotides
- Osmium Tetroxide
- Plasmids
- Restriction Mapping
- Transformation, Bacterial