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Article

Mutant profiles of selectable genetic elements.

Proceedings of the National Academy of Sciences of the United States of America - 15 Nov 1991

Wurst H, Pohl F M

Abstract excerpt

A method is presented that allows simultaneous analysis of the effects of all possible point mutations within a specific mutation window of at least 50 base pairs on a DNA fragment that codes for a selectable function. It relies on the detection of mismatched base pairs with hydroxylamine and osmium tetroxide. A mutant plasmid library of randomly distributed point mutations within the lacZ' gene of Escherichia...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 1946459
DOI: 10.1073/pnas.88.22.9909
PMCID: PMC52836

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