Article
Linker mutagenesis in the gene encoding the periplasmic maltose-binding protein of E. coli.
Biochimie - 1 Jan 2000
Duplay P, Bedouelle H, Szmelcman S, Hofnung M
Abstract excerpt
A plasmid carrying the malE gene, coding for the periplasmic maltose-binding protein of E. coli, was submitted to random mutagenesis by the insertion of a BamHI linker. About 25% of the clones recovered had acquired a BamHI site in the gene malE. Most of the linker insertions were accompanied by small deletions with an average size of 30 base pairs. Among 21 mutants synthesizing a stable maltose binding protein,...
Topics
- ATP-Binding Cassette Transporters
- Carrier Proteins
- DNA Restriction Enzymes
- DNA Transposable Elements
- Escherichia coli
- Escherichia coli Proteins
- Genes
- Genes, Bacterial
- Genetic Linkage
- Maltose-Binding Proteins
- Membrane Proteins