Genetic characterization of the vaccinia virus DNA polymerase: identification of point mutations conferring altered drug sensitivities and reduced fidelity.

doi:10.1128/JVI.65.2.869-879.1991

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Article

Genetic characterization of the vaccinia virus DNA polymerase: identification of point mutations conferring altered drug sensitivities and reduced fidelity.

Journal of virology - 1 Feb 1991

Taddie J A, Traktman P

Abstract excerpt

We determined that 85 microM aphidicolin was sufficient to block macroscopic plaque formation by vaccinia virus and to cause a 10(4)-fold reduction in viral yield from a wild-type infection. A chemically mutagenized viral stock was passaged sequentially in the presence of drug, and plaque-purified viral stocks resistant to aphidicolin were isolated and characterized. By use of a marker rescue protocol, the lesion...

Topics

Amino Acid Sequence
Animals
Aphidicolin
Base Sequence
Cell Line
DNA, Viral
DNA-Directed DNA Polymerase
Diterpenes
Drug Resistance, Microbial
Genes, Viral
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutagenesis, Site-Directed

Mutation

Restriction Mapping

Vaccinia virus

Viral Plaque Assay

Viral Structural Proteins

Virus Replication

Source

Open in PubMed Open full text

Identifiers

PMID: 1898973
DOI: 10.1128/JVI.65.2.869-879.1991
PMCID: PMC239827

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1898973-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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