Article
A PCR-based method for manipulation of the vaccinia virus genome that eliminates the need for cloning.
BioTechniques - 1 Nov 1992
Turner P C, Moyer R W
Abstract excerpt
A general method is described for altering specific genes of vaccinia virus (VV). We demonstrate and evaluate the procedure by gene inactivation, using a dominant selectable marker in conjunction with recombinant polymerase chain reaction (PCR). Primers based on the sequence of the target gene enable amplification of flanking arms and their subsequent attachment to the gpt cassette that confers resistance to...
Topics
- Base Sequence
- Blotting, Southern
- Cloning, Molecular
- DNA, Single-Stranded
- Genetic Techniques
- Genome, Viral
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- Thymidine Kinase
- Transfection