Article
Altered pore properties and kinetic changes in mutants of the Vibrio cholerae porin OmpU.
Molecular membrane biology - 1 Sept 2008
Lauman Brian, Pagel Melissa, Delcour Anne H
Abstract excerpt
The electrophysiological technique of patch-clamp was used to characterize the pore properties of site-directed mutants in the Vibrio cholerae general diffusion porin OmpU. Changes in conductance and selectivity were observed, thus confirming the predicted pore location of these residues, based on homology with the Escherichia coli porins OmpF and OmpC. Some mutants acquire a weak selectivity for cations, which...
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