Article
Selectivity changes in site-directed mutants of the VDAC ion channel: structural implications.
Science (New York, N.Y.) - 9 Mar 1990
Blachly-Dyson E, Peng S, Colombini M, Forte M
Abstract excerpt
The gene encoding the yeast mitochondrial outer membrane channel VDAC was subjected to site-directed mutagenesis to change amino acids at 29 positions to residues differing in charge from the wild-type sequence. The mutant genes were then expressed in yeast, and the physiological consequences of...
Topics
- Amino Acid Sequence
- Cloning, Molecular
- Intracellular Membranes
- Ion Channels
- Lipid Bilayers
- Membrane Potentials
- Membrane Proteins
- Mitochondria
- Molecular Sequence Data
- Mutation
- Porins
- Protein Conformation
- Saccharomyces cerevisiae
- Structure-Activity Relationship
- Voltage-Dependent Anion Channels