A single amino acid substitution alters conductance and gating of OmpC porin of Escherichia coli.

doi:10.1007/BF01868731

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Article

A single amino acid substitution alters conductance and gating of OmpC porin of Escherichia coli.

The Journal of membrane biology - 1 Feb 1991

Delcour A H, Adler J, Kung C

Abstract excerpt

We have reconstituted into liposomes outer-membrane fractions from Escherichia coli strains which express OmpC porins with altered pore properties. Single-channel experiments were performed with the patch-clamp technique on blisters generated from the reconstituted liposomes. Our goal was to iden...

Topics

Bacterial Outer Membrane Proteins
Electric Conductivity
Escherichia coli
Ion Channel Gating
Liposomes
Membrane Potentials
Mutation

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