Article
Limited regions of the alpha 2-domain alpha-helix control anti-A2 allorecognition: an analysis using a panel of A2 mutants.
Immunogenetics - 1 Jan 1991
Lombardi G, Matsui M, Moots R, Aichinger G, Sidhu S, Batchelor R, Frelinger J, Lechler R
Abstract excerpt
The regions of the HLA-A2 molecule controlling anti-A2 alloreactivity were explored using naturally occurring allelic variants of HLA-A, and a panel of transfectants expressing the products of A2.1 genes that had been mutated at multiple positions encoding residues in the alpha 2 domain alpha-helix. As a means of detecting distant conformational effects, these altered A2.1 molecules were also examined...
Topics
- Cell Line
- HLA-A2 Antigen
- Humans
- Mutation
- Protein Conformation
- Structure-Activity Relationship
- T-Lymphocytes
- Transfection