Article
Subtractive hybridization cloning of a tissue-specific extinguisher: TSE1 encodes a regulatory subunit of protein kinase A.
Cell - 6 Sept 1991
Jones K W, Shapero M H, Chevrette M, Fournier R E
Abstract excerpt
Tissue-specific extinguisher 1 (TSE1) is a trans-acting locus on human chromosome 17 that down-regulates expression of seven liver genes in hepatoma x fibroblast hybrids. To study the mechanism by which TSE1 functions, we used subtractive cDNA hybridization to clone transcripts encoded within a 2...
Topics
- Base Sequence
- Chromosomes, Human, Pair 17
- Cloning, Molecular
- Cyclic AMP-Dependent Protein Kinase RIalpha Subunit
- Cyclic AMP-Dependent Protein Kinases
- DNA
- Gene Expression Regulation
- Humans
- Molecular Sequence Data
- Nucleic Acid Hybridization
- Oligonucleotides
- Phenotype
- Polymerase Chain Reaction
- Protein Kinases
- Proteins
- Receptors, Cyclic AMP
- Sequence Homology, Nucleic Acid
- Transfection