Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Exon sequence and structure requirements for tRNA splicing in Saccharomyces cerevisiae.

Biochemistry - 3 Mar 1992

Shapero M H, Greer C L

Abstract excerpt

A survey of exon sequence and structure requirements for splicing was undertaken using labeled pre-tRNA substrates prepared by in vitro transcription of bacterial promoter-yeast tRNA(Tyr) gene fusions. Transcription templates were assembled from oligonucleotide cassettes allowing analysis of 22 derivatives affecting each of the potential secondary and certain tertiary interactions in the pre-tRNA. Effects on both...

Topics

Base Sequence
Exons
Genes, Fungal
Molecular Sequence Data
Mutation
Nucleic Acid Conformation
RNA Splicing
RNA, Fungal
RNA, Transfer
RNA, Transfer, Phe
RNA, Transfer, Ser
RNA, Transfer, Tyr

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Saccharomyces cerevisiae

Templates, Genetic

Transcription, Genetic

Source

Identifiers

PMID: 1540592
DOI: 10.1021/bi00123a022

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1540592-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Exon sequence and structure requirements for tRNA splicing in Saccharomyces cerevisiae.PMID 1540592Read ↗

Use of RNase P for efficient preparation of yeast tRNATyr transcript and its mutants.Semantic proximity Read ↗

Splice junction mutations in a yeast tRNA gene which alter the rate and precision of processing.Semantic proximity Read ↗

Pleiotropic effect of a point mutation in the yeast SUP4-o tRNA gene: in vivo pre-tRNA processing in S. cerevisiae.Semantic proximity Read ↗

Measurement and analysis of yeast pre-mRNA sequence contribution to splicing efficiency.Semantic proximity Read ↗

Analysis of mutant tRNA gene transcripts in vivo in Saccharomyces cerevisiae by abortive primer extension.Semantic proximity Read ↗

Plant nonsense suppressor tRNA(Tyr) genes are expressed at very low levels in vitro due to inefficient splicing of the intron-containing pre-tRNAs.Semantic proximity Read ↗

Deletion analysis of a multifunctional yeast tRNA ligase polypeptide. Identification of essential and dispensable functional domains.Semantic proximity Read ↗

Enzymatic formation of modified nucleosides in tRNA: dependence on tRNA architecture.Semantic proximity Read ↗

Construction of an opal suppressor by oligonucleotide-directed mutagenesis of a Saccharomyces cerevisiae tRNA(Trp) gene.Semantic proximity Read ↗

tRNA determinants for transcription antitermination of the Bacillus subtilis tyrS gene.Semantic proximity Read ↗

The 5' end of yeast 5.8S rRNA is generated by exonucleases from an upstream cleavage site.Semantic proximity Read ↗

In vitro genetic analysis of the structural features of the pre-tRNA required for determination of the 3' splice site in the intron excision reaction.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.