Article
Exon sequence and structure requirements for tRNA splicing in Saccharomyces cerevisiae.
Biochemistry - 3 Mar 1992
Shapero M H, Greer C L
Abstract excerpt
A survey of exon sequence and structure requirements for splicing was undertaken using labeled pre-tRNA substrates prepared by in vitro transcription of bacterial promoter-yeast tRNA(Tyr) gene fusions. Transcription templates were assembled from oligonucleotide cassettes allowing analysis of 22 derivatives affecting each of the potential secondary and certain tertiary interactions in the pre-tRNA. Effects on both...
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