Article
Purines are required at the 5' ends of newly initiated RNAs for optimal RNA polymerase III gene expression.
Molecular and cellular biology - 1 Oct 1996
Zecherle G N, Whelen S, Hall B D
Abstract excerpt
We have made specific alterations in the CAACAA element at the transcription start site of a Saccharomyces cerevisiae suppressor tRNA gene. The mutant genes were tested for their ability to suppress the ochre nonsense alleles ade2-1, lys4-1, and met4-1. Many of the mutants showed either no phenotypic change or a weak loss of suppression relative to that of SUP4-o. A 2-bp change, CTCCAA, which alters bases...
Topics
- Alleles
- Base Sequence
- Fungal Proteins
- Gene Expression Regulation, Enzymologic
- Kinetics
- Mutagenesis, Site-Directed
- Peptide Termination Factors
- Phenotype
- Prions
- Purines
- RNA Polymerase III
- RNA, Fungal
- RNA, Transfer
- Restriction Mapping
- Saccharomyces cerevisiae